Western blot analysis of extracts of Rat spleen, using Anti-IRF1 Antibody (A15842) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western blot analysis of extracts of C2C12 cells, using Anti-IRF1 Antibody (A15842) at 1:1,000 dilution. C2C12 cells were treated by IFNgamma (50ng/ml) for 8 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-IRF1 Antibody (A15842) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
