Recombinant chimeric rabbit monoclonal antibody to PD1.
Applications
ELISA, Flow Cytometry
Dilutions
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reactivity
Canine
Host
Chimeric Rabbit
Clonality
Monoclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Synonyms
CD279, PDCD1, Programmed cell death protein 1, Protein PD-1
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
ELISA plates were pre-coated with Recombinant Canine PD1 Protein (10xHis Tag) (A324898) at 2 µg/ml (100 µl/well) which can bind INTERVET 4F12 Biosimilar - Anti-PD1 Antibody - BSA and Azide free (A324674) in a linear range of 0.64-80 ng/ml.
ELISA plates were pre-coated with Recombinant Canine PD1 Protein (Fc Tag) (A324900) at 2 µg/ml (100 µl/well) which can bind INTERVET 4F12 Biosimilar - Anti-PD1 Antibody - BSA and Azide free (A324674) in a linear range of 0.64-80 ng/ml.
ELISA plates were pre-coated with Recombinant Canine PD1 Protein (Fc Tag) (A324899) at 2 µg/ml (100 µl/well) which can bind INTERVET 4F12 Biosimilar - Anti-PD1 Antibody - BSA and Azide free (A324674) in a linear range of 0.64-80 ng/ml.
Flow cytometry analysis of 1µg/ml of INTERVET 4F12 Biosimilar - Anti-PD1 Antibody - BSA and Azide free (A324674) on Expi293 cells transfected with Canine PD-1 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
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