Flow cytometry analysis of viable equine PBMC using Anti-Interferon gamma Antibody [MT166] (PF488P). Cells were stimulated for 16 hours in the presence of PMA/ionomycin and Brefeldin A, and then fixed and permeabilized using 4% paraformaldehyde and saponin, and subsequently stained with Anti-Interferon gamma Antibody [MT166] (PF488P) (solid line) or isotype control antibody (dashed line). The histogram derives from gated events of typical lymphocyte characteristics in forward and side light scatter.
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