Anti-Interferon gamma Antibody - BSA and Azide free (A283973)

Anti-Interferon gamma Antibody - BSA and Azide free

Rabbit polyclonal antibody to Interferon gamma.

This antibody recognises chicken interferon gamma (chIFN-gamma). There are at least three distinct chicken interferon proteins, designated as chIFN-alpha, chIFN-beta and chIFN-gamma.chIFN-gamma was first identified, when supernatant from chicken spleen cells stimulated with concanavalin A (ConA ), displayed macrophage-activating factor (MAF) activity - the principle function of mammalian type II IFN-gamma. Cloning of IFN-gamma cDNA from activated chicken T cells indicates that recombinant chicken IFN-gamma activates macrophages, strongly upregulating the expression of MHC class II and iNOS genes. Rabbit anti Chicken interferon gamma antibody can be used to neutralize chIFN-gamma activity and to discriminate the antiviral activity of chIFN-gamma from that of chIFN-alpha and chIFN-beta.

ELISA, Functional Assays, WB

Functional Assays: 10 µg/ml - 50 µg/ml

Chicken

Recombinant Chicken interferon gamma.

Rabbit

Polyclonal

IgG

Approximately 18 kDa.

Liquid

Supplied in Phosphate Buffered Saline.

Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

Rabbit anti Chicken interferon gamma antibody recognizes chicken interferon gamma (chIFN-gamma). There are at least three distinct chicken interferon proteins, designated as chIFN-alpha, chIFN-beta and chIFN-gamma.chIFN-gamma was first identified, when supernatant from chicken spleen cells stimulated with concanavalin A (ConA ), displayed macrophage-activating factor (MAF) activity - the principle function of mammalian type II IFN-gamma (Vonbulow et al. 1984). Cloning of IFN-gamma cDNA from activated chicken T cells indicates that recombinant chicken IFN-gamma activates macrophages, strongly upregulating the expression of MHC class II and iNOS genes (Weining et al. 1996).Rabbit anti Chicken interferon gamma antibody can be used to neutralize chIFN-gamma activity and to discriminate the antiviral activity of chIFN-gamma from that of chIFN-alpha and chIFN-beta (Weining et al. 1996).