Mouse monoclonal [4S.B3] antibody to Interferon gamma (PerCP-Cyanine 5.5).
Specificity
This antibody recognizes Interferon gamma, a 16-25 kDa cytokine produced by activated Th1 cells and NK cells. This antibody binds both glycosylated and non-glycosylated Interferon gamma.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests; Intracellular staining.
Reactivity
Human, Non-Human Primates
Immunogen
Interferon gamma derived from human leukocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
4S.B3
Isotype
IgG1
Light Chains
kappa
Conjugate
PerCP-Cyanine 5.5
Excitation: 482nm, Emission: 695nm
Purification
This antibody is conjugated with tandem dye PerCP-Cyanine 5.5 under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
16-25 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Flow cytometric analysis of Interferon gamma in PHA activated human PBMC with Anti-Interferon gamma Antibody [4S.B3] (PerCP-Cyanine 5.5).
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