Rat monoclonal [5H10-27 (MFR5)] antibody to Integrin alpha 5 (PE).
Specificity
This antibody recognizes an extracellular epitope of murine CD49e, a 135 kDa protein serving as VLA-5 alpha chain, expressed on thymocytes, activated T cells, splenic B cells, and mast cells.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 1-4 µg/ml.
Reactivity
Mouse
Immunogen
(C57BL/6 x A/J)F1 murine mast cell line.
Host
Rat
Clonality
Monoclonal
Clone ID
5H10-27 (MFR5)
Isotype
IgG2a
Light Chains
kappa
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Concentration
500 µg/ml
Predicted MW
135 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
CD49 antigen-like family member E, CD49e, Fibronectin receptor subunit alpha, FNRA, Integrin alpha-5, Integrin alpha-F, ITGA5, VLA-5
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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