Anti-Integrin alpha 2 Antibody [DX5] (FITC) (A280281)

Anti-Integrin alpha 2 Antibody [DX5] (FITC)

Rat monoclonal [DX5] antibody to Integrin alpha 2 (FITC).

This antibody recognises mouse CD49b, also known as Very Late Antigen-2 (VLA-2) or alpha-2 integrin. CD49b is a ~160kDa single pass type I membrane protein expressed by NK cells and a small subset of T-cells. Rat anti mouse CD49b, clone DX5 stains NK cells from all mouse strains tested including Balb/c mice. CD49b acts as a receptor for collagen, laminin, fibronectin and E-cadherin.

Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl. The Fc region of monoclonal antibodies may bind to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/BUF041B).

Mouse

RIL-2 propagated NK1.1+ cells from C57BL/6 mice.

Rat

Monoclonal

IgM

100 µg/ml

Liquid

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Store undiluted at 4°C only. Do not freeze! This product is photosensitive and should be protected from light. Avoid freeze / thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

Rat anti Mouse CD49b monoclonal antibody, clone DX5 recognizes mouse CD49b, also known as Very Late Antigen-2 (VLA-2) or alpha-2 integrin. CD49b is a ~160kDa single pass type I membrane protein expressed by NK cells and a small subset of T-cells (Pellicci et al. 2005). Rat anti mouse CD49b, clone DX5 stains NK cells from all mouse strains tested including Balb/c mice (Arese et al. 2001). CD49b acts as a receptor for collagen, laminin, fibronectin and E-cadherin (Carafoli et al. 2013).

CD49 antigen-like family member B, CD49b, Collagen receptor, GPIa, Integrin alpha-2, ITGA2, Platelet membrane glycoprotein Ia, VLA-2 subunit alpha

This product is for research use only. It is not intended for diagnostic or therapeutic use.