Anti-Integrin alpha 2 Antibody [ARC0457] (A306130)

Western blot analysis of extracts from wild type(WT) and Integrin alpha 2 (ITGA2/CD49b) knockout (KO) 293T cells, using Anti-Integrin alpha 2 Antibody [ARC0457] (A306130) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Integrin alpha 2 Antibody [ARC0457] (A306130) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunoprecipitation analysis of 300µg extracts of A-549 cells using 3µg of Anti-Integrin alpha 2 Antibody [ARC0457] (A306130). This Western blot was performed on the immunoprecipitate using Anti-Integrin alpha 2 Antibody [ARC0457] (A306130) at a dilution of 1:1000.