Anti-Integrin alpha 2 Antibody (A10175)

Name: Anti-Integrin alpha 2 Antibody
Brand: Antibodies.com
SKU: A10175
Price: 485.0 USD
Availability: InStock

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$485

Rabbit polyclonal antibody to Integrin alpha 2 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Tags:

Knockout Validated Antibody

Specifications

Name

Anti-Integrin alpha 2 Antibody

Description

Rabbit polyclonal antibody to Integrin alpha 2.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Integrin alpha 2 (ITGA2/CD49b) (NP_002194.2).

Sequence

MGPERTGAAPLPLLLVLALSQGILNCCLAYNVGLPEAKIFSGPSSEQFGYAVQQFINPKGNWLLVGSPWSGFPENRMGDVYKCPVDLSTATCEKLNLQTS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

150 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CD49 antigen-like family member B, CD49b, Collagen receptor, GPIa, Integrin alpha-2, ITGA2, Platelet membrane glycoprotein Ia, VLA-2 subunit alpha

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (6)

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Western Blot - Anti-Integrin alpha 2 Antibody (A10175)

Western blot analysis of extracts of various cell lines, using Anti-Integrin alpha 2 Antibody (A10175) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Western Blot - Anti-Integrin alpha 2 Antibody (A10175)

Western blot analysis of extracts from normal (control) and Integrin alpha 2 (ITGA2/CD49b) knockout (KO) 293T cells, using Anti-Integrin alpha 2 Antibody (A10175) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Immunohistochemistry - Anti-Integrin alpha 2 Antibody (A10175)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-Integrin alpha 2 Antibody (A10175) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Integrin alpha 2 Antibody (A10175)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-Integrin alpha 2 Antibody (A10175) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.