Anti-Integrin alpha 2 Antibody [16B4] (A279044)

Anti-Integrin alpha 2 Antibody [16B4]

Mouse monoclonal [16B4] antibody to Integrin alpha 2.

This antibody recognises human CD49b, also known as integrin a2, collagen receptor, Platelet membrane glycoprotein Ia or VLA-2 subunit alpha. CD49b is a 1181 amino acid ~160 kDa single pass type-1 transmembrane glycoprotein possessing multiple FG-GAP repeats and a single VWFA domain. Mouse anti human CD49b antibody, clone 16B4 is identified as capable of immunoprecipitating a non-reducible alpha subunit from I125 surface labeled human cell line extract. It is confirmed as specific to the alpha 2 subunit by relative expression of antigen on various cell lines by FACS, and its recognition of affinity purified alpha 2 beta 1 in dot blots.

WB, IHC-P, IP, Flow Cytometry, IHC-Fr, ELISA, Functional Assays, IF

ELISA: 10 µg/ml, Flow Cytometry: 30 µg/ml, IHC-Fr: 30 µg/ml - 40 µg/ml, IP: 25 µg/ml - 50 µg/ml

Human

This antibody does not cross react with Rat

Purified human beta 1 preparation from HT1080 fibrosarcoma cell extract.

Mouse

Monoclonal

16B4

IgG1

Protein G affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human CD49b antibody, clone 16B4 recognizes human CD49b, also known as integrin a2, collagen receptor, Platelet membrane glycoprotein Ia or VLA-2 subunit alpha. CD49b is a 1181 amino acid ~160 kDa single pass type-1 transmembrane glycoprotein possessing multiple FG-GAP repeats and a single VWFA domain. Mouse anti human CD49b antibody, clone 16B4 is identified as capable of immunoprecipitating a non-reducible alpha subunit from I125 surface labeled human cell line extract. It is confirmed as specific to the alpha 2 subunit by relative expression of antigen on various cell lines by FACS, and its recognition of affinity purified alpha 2 beta 1 in dot blots.

CD49 antigen-like family member B, CD49b, Collagen receptor, GPIa, Integrin alpha-2, ITGA2, Platelet membrane glycoprotein Ia, VLA-2 subunit alpha