Recombinant humanized monoclonal antibody to Claudin 6.
Applications
ELISA, Flow Cytometry
Dilutions
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reactivity
Human
Host
Humanized
Clonality
Monoclonal
Isotype
IgG1
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Flow cytometry analysis of 1µg/ml of IMAB027 Biosimilar - Anti-Claudin 6 Antibody - BSA and Azide free (A318880) on Expi293 cells transfected with Human CLDN6 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
ELISA plates were pre-coated with 0.5 µg/well purified human CLDN6 MNPs. Serial diluted IMAB027 Biosimilar - Anti-Claudin 6 Antibody - BSA and Azide free (A318880) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From the above data, the EC50 for IMAB027 Biosimilar - Anti-Claudin 6 Antibody - BSA and Azide free (A318880) binding with Claudin6 MNPs is 34.36 ng/ml.
Flow cytometry analysis of 15µg/ml of IMAB027 Biosimilar - Anti-Claudin 6 Antibody - BSA and Azide free (A318880) on Expi293 cells transfected with Human CLDN9 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
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