Recombinant human monoclonal [CSL311] antibody to IL-3RB.
Applications
ELISA, FACS, Functional Assay, In Vivo
Reactivity
Human
Host
Human
Clonality
Monoclonal
Clone ID
CSL311
Isotype
IgG4
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A affinity chromatography.
Concentration
Reconstitution dependent.
Molecular Weight
This antibody has a predicted MW of 144.66 kDa.
Purity
> 95% (by SDS-PAGE and SEC-HPLC).
Product Form
Lyophilized
Reconstitution
Reconstitute with 100µl of sterile double-distilled water to bring antibody to 1mg/ml concentration. Gently shake to solubilize completely. Do not vortex!
Formulation
Lyophilized from 25mM Histadine, pH 6.2, with 8% Sucrose and 0.01% Tween80.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
SDS-PAGE analysis of Anti-IL-3RB Antibody [CSL311] - Low endotoxin, Azide free (A324093) under reducing (R) conditions confirms that the purity of this antibody is greater than 95%.
Human CSF2RB HEK293 cells were stained with Anti-IL-3RB Antibody [CSL311] - Low endotoxin, Azide free (A324093) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50 = 0.049 µg/ml.
IL-3-mediated proliferation of TF-1 can be completely blocked by Anti-IL-3RB Antibody [CSL311] - Low endotoxin, Azide free (A324093) with an IC50 of 0.2109 nM.
GM-CSF-mediated proliferation of TF-1 can be completely blocked by Anti-IL-3RB Antibody [CSL311] - Low endotoxin, Azide free (A324093) with an IC50 of 4.105 nM.
Publishing research using Recombinant Anti-IL-3RB Antibody [CSL311] - Low endotoxin, Azide free (A324093)? Please let us know so that we can list the citation on this page.