Anti-IL-12B Antibody [D14-5G11] (A280208)

Anti-IL-12B Antibody [D14-5G11]

Mouse monoclonal [D14-5G11] antibody to IL-12B.

This antibody recognises interleukin-12 p40 (IL-12 p40) also known as interleukin-12 subunit beta and cytotoxic lymphocyte maturation factor 40 kDa subunit. IL-12 is a heterodimeric cytokine composed of 2 subunits, a 35 kDa subunit (p35) and a 40 kDa subunit (p40). The IL-12 p40 subunit is largely expressed by dendritic cells, monocytes, macrophages, neutrophils, B-lymphocytes and keratinocytes. It is able to form a homodimer, which was believed to be inactive. However it has subsequently been demonstrated to have proinflammatory properties through its induction of inducible nitric oxide synthase in microglia (Pahan et al. 2001, Jana et al. 2009).IL-12 p40 coupled to the protein p19 forms the heterodimer IL-23 (p19-p40), when this formation takes place in activated dentritic cells the p19-p40 binds to its receptor IL-12Rß1 to activate the STAT4 signaling pathway. This results in the stimulation of IFN? production along with proliferation of PHA blast T cells. The purified Mouse anti Human interleukin-12 p40 antibody, clone D14-5G11 (MCA6194GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human interleukin-12 p40 antibody, clone M18 (MCA6195B) as the detection antibody.

ELISA: 1 µg/ml

Human

E. coli derived recombinant human full length interleukin-12 p40 (Ile23-Ser328).

Mouse

Monoclonal

D14-5G11

IgG1

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human Interleukin-12 p40 antibody, clone D14-5G11, recognizes interleukin-12 p40 (IL-12 p40) also known as interleukin-12 subunit beta and cytotoxic lymphocyte maturation factor 40 kDa subunit. IL-12 is a heterodimeric cytokine composed of 2 subunits, a 35 kDa subunit (p35) and a 40 kDa subunit (p40). The IL-12 p40 subunit is largely expressed by dendritic cells, monocytes, macrophages, neutrophils, B-lymphocytes and keratinocytes (Airoldi et al. 2002). It is able to form a homodimer, which was believed to be inactive. However it has subsequently been demonstrated to have proinflammatory properties through its induction of inducible nitric oxide synthase in microglia (Pahan et al. 2001, Jana et al. 2009). IL-12 p40 coupled to the protein p19 forms the heterodimer IL-23 (p19-p40), when this formation takes place in activated dentritic cells the p19-p40 binds to its receptor IL-12Rß1 to activate the STAT4 signaling pathway. This results in the stimulation of IFN? production along with proliferation of PHA blast T cells (Airoldi et al. 2002).The purified Mouse anti Human interleukin-12 p40 antibody, clone D14-5G11 (MCA6194GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human interleukin-12 p40 antibody, clone M18 (MCA6195B) as the detection antibody.

CLMF p40, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, IL12B, Interleukin-12 subunit beta, NK cell stimulatory factor chain 2, NKSF2