Anti-IL-10 Antibody - BSA and Azide free (A283937)

Anti-IL-10 Antibody - BSA and Azide free

Rabbit polyclonal antibody to IL-10.

This antibody recognises human IL-10 (Interleukin-10), an 18.5kDa immunosuppressive cytokine originally known as cytokine synthesis inhibiting factor (CSIF), which specifically regulates immune-mediated inflammation and shares high sequence homology with murine IL-10 and the viral protein BCRF1.IL-10, produced by macrophages, mast cells, T cells, activated B cells and keratinocytes, inhibits the secretion of a variety of pro-inflammatory cytokines including TNFalpha, IL-1, IL-2, IL-3 and IL-8 and is chemotactic for CD8+ T cells. The suppressive ability of IL-10 has become a focus area for the treatment of Crohn’s disease, but with conflicting results.

WB, ELISA, Functional Assays

ELISA: 0.5 µg/ml, Functional Assays: 4.0 - 6.0 µg/ml, WB: 0.1 - 0.2 µg/ml

Human

Recombinant human IL-10.

Rabbit

Polyclonal

IgG

1 mg/ml after reconstitution.

Reconstitute with 0.1 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. For functional studies do not add sodium azide.

Supplied in Phosphate Buffered Saline.

Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Rabbit anti Human Interleukin-10 antibody recognizes human IL-10 (Interleukin-10), an 18.5kDa immunosuppressive cytokine originally known as cytokine synthesis inhibiting factor (CSIF), which specifically regulates immune-mediated inflammation and shares high sequence homology with murine IL-10 and the viral protein BCRF1.IL-10, produced by macrophages, mast cells, T cells, activated B cells and keratinocytes, inhibits the secretion of a variety of pro-inflammatory cytokines including TNFalpha, IL-1, IL-2, IL-3 and IL-8 and is chemotactic for CD8+ T cells. The suppressive ability of IL-10 has become a focus area for the treatment of Crohn’s disease, but with conflicting results.

CSIF, Cytokine synthesis inhibitory factor, IL10, Interleukin-10