Western Blot - Anti-IKB alpha (phospho Ser36) Antibody (A306545)
Western blot analysis of extracts of HeLa cells, using Anti-IKB alpha (phospho Ser36) Antibody (A306545) at 1:1,000 dilution or IKB alpha antibody (A11397). HeLa cells were treated by TNF-alpha (20 ng/ml) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 30s.
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