This antibody recognises NF-kappa-B inhibitor alpha (NFkBIa, also known as IkB alpha). NFkBIa is one of seven members of the IkB class of inhibitor proteins that have been identified to date. NFkBIa inhibits the NFkB complex in unstimulated cells through non-covalent binding. Following stimulation of cells, NFkBIa is phosphorylated so that it dissociates from the NFkB and then ubiquinated prior to degradation.
Applications
WB, ELISA, IP, Gel Super Shift Assays
Dilutions
ELISA: 1:2,000, WB: 1:200 - 1:1,000
Reactivity
Human
Immunogen
Human NF kappa B inhibitor alpha expressed in E. coli.
Host
Sheep
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Concentration
1 mg/ml
Molecular Weight
Approximately 39 kDa in THPI cell lysates.
Product Form
Liquid
Formulation
Supplied in TRIS Buffered Saline with <0.1% Sodium Azide and 0.5% BSA.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Sheep anti Human NF Kappa B Inhibitor alpha antibody recognizes NF-kappa-B inhibitor alpha (NFkBIa, also known as IkB alpha). NFkBIa is one of seven members of the IkB class of inhibitor proteins that have been identified to date. NFkBIa inhibits the NFkB complex in unstimulated cells through non-covalent binding. Following stimulation of cells, NFkBIa is phosphorylated so that it dissociates from the NFkB and then ubiquinated prior to degradation.
Synonyms
I-kappa-B-alpha, IkappaBalpha, IkB-alpha, IKBA, MAD3, Major histocompatibility complex enhancer-binding protein MAD3, NF-kappa-B inhibitor alpha, NFKBI, NFKBIA