IGFBP3 expression in Human Breast Cancer lysate with (B) and without (A) immunising peptide blockage analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-IGFBP3 Antibody (A83357) at 0.02µg/ml and detected by chemiluminescence.
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