Anti-IGF1 Receptor Antibody [DMC721] - BSA and Azide free
Description
Recombinant chimeric monoclonal [DMC721] antibody to IGF1 Receptor.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 1:100
Reactivity
Human
Host
Rabbit / Human
Clonality
Monoclonal
Clone ID
DMC721
Isotype
IgG1
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
This antibody is a recombinant rabbit chimeric monoclonal antibody containing human IgG1 Fc.
(A) Anti-IGF1 Receptor Antibody [DMC721] - BSA and Azide free (A324651) does not bind to Jurkat cells that do not express IGF-1R. (B) A clear peak shift of Anti-IGF1 Receptor Antibody [DMC721] - BSA and Azide free (A324651) was seen compared to the control when incubated with IGF-1R-expressing MCF-7 cells, indicating strong binding of Anti-IGF1 Receptor Antibody [DMC721] - BSA and Azide free (A324651) to IGF-1R. Antibodies were incubated at 5 µg/ml.
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