Western blot analysis of Jurkat, using Anti-IDH2 Antibody (A15584) at 1:600 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Western blot analysis of various lysates, using Anti-IDH2 Antibody (A15584) at 1:600 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-IDH2 Antibody (A15584) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunoprecipitation analysis of 200µg extracts of MCF7 cells using 1µg of Anti-IDH2 Antibody (A15584). This Western blot was performed on the immunoprecipitate using Anti-IDH2 Antibody (A15584) at a dilution of 1:1000.
Chromatin immunoprecipitation (ChIP) analysis of extracts of 293T cell line, using Anti-IDH2 Antibody (A15584) and Rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
