IDH1 expression in Saccharomyces cerevisiae S288c lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-IDH1 Antibody (A84925) at 0.5µg/ml and detected by chemiluminescence. Note the blocking buffer contains non-animal derived protein.
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