Anti-ICAM1 Antibody [84H10] (A279533)

$340

Mouse monoclonal [84H10] antibody to ICAM1 for ELISA, Functional Assays, Flow Cytometry, IHC-Fr and IP.

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 4-6 business days.
Name
Anti-ICAM1 Antibody [84H10]
Description
Mouse monoclonal [84H10] antibody to ICAM1.
Specificity
This antibody recognises the D1 domain of ICAM-1.It reacts with the ICAM-1 antigen found in low levels on lymphocytes and strongly expressed on monocytes and granulocytes. This molecule is inducible to high levels by mitogenic lectins on lymphocytes and by IL-1 beta or IFN gamma on other cell types such as fibroblasts and endothelial cells. Mouse anti Human CD54 antibody, clone 84H10 detects an antigen of ~90 kDa. Mouse anti Human CD54 antibody, clone 84H10 has been reported to block ICAM1 mediated cellular adhesion and block binding of LFA-1 and P. falciparum to ICAM-1. Mouse anti Human CD54 antibody, clone 84H10 is routinely tested in flow cytometry on rat splenocytes.
Applications
ELISA, Functional Assays, Flow Cytometry, IHC-Fr, IP
Dilutions
Flow Cytometry: Neat - 1:10, IHC-Fr 2: 1:20 - 1:50, IP: 5µg per 107 cells, Use 10µl of the suggested working dilution to label 106 cells in 100µl.
Reactivity
Human, Dog
Immunogen
K562 cell line.
Host
Mouse
Clonality
Monoclonal
Clone ID
84H10
Isotype
IgG1
Conjugate

Unconjugated

Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Human CD54 antibody, clone 84H10 recognizes the D1 domain of ICAM-1.It reacts with the ICAM-1 antigen found in low levels on lymphocytes and strongly expressed on monocytes and granulocytes. This molecule is inducible to high levels by mitogenic lectins on lymphocytes and by IL-1 beta or IFN gamma on other cell types such as fibroblasts and endothelial cells. Mouse anti Human CD54 antibody, clone 84H10 detects an antigen of ~90 kDa.Mouse anti Human CD54 antibody, clone 84H10 has been reported to block ICAM1 mediated cellular adhesion and block binding of LFA-1 and P. falciparum to ICAM-1.Mouse anti Human CD54 antibody, clone 84H10 is routinely tested in flow cytometry on rat splenocytes.
Synonyms
ICAM-1, Intercellular adhesion molecule 1, Major group rhinovirus receptor
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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