Anti-Iba1 Antibody [ARC2301] (A307823)

Name: Anti-Iba1 Antibody [ARC2301]
Brand: Antibodies.com
SKU: A307823
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC2301] antibody to Iba1 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

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Specifications

Name

Anti-Iba1 Antibody [ARC2301]

Description

Rabbit monoclonal [ARC2301] antibody to Iba1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of mouse AIF1/IBA1 (O70200).

Sequence

MSQSRDLQGGKAFGLLKAQQEERLEGINKQFLDDPKYSNDEDLPSKLEAFKVKYMEFDLNGNGDIDIMSLKRMLEKLGVPKTHLELKRLIREVSSGSEET

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2301

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

17 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AIF-1, AIF1, Allograft inflammatory factor 1, G1, Ionized calcium-binding adapter molecule 1, Protein G1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (7)

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Western Blot - Anti-Iba1 Antibody [ARC2301] (A307823)

Western blot analysis of extracts of various cell lines, using Anti-Iba1 Antibody [ARC2301] (A307823) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Immunohistochemistry - Anti-Iba1 Antibody [ARC2301] (A307823)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Iba1 Antibody [ARC2301] (A307823) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Iba1 Antibody [ARC2301] (A307823)

Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-Iba1 Antibody [ARC2301] (A307823) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Iba1 Antibody [ARC2301] (A307823)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-Iba1 Antibody [ARC2301] (A307823) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.