Anti-Iba1 Antibody (A13488)

Name: Anti-Iba1 Antibody
Brand: Antibodies.com
SKU: A13488
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Iba1 for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

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Microglia Marker

Specifications

Name

Anti-Iba1 Antibody

Description

Rabbit polyclonal antibody to Iba1.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human AIF1/IBA1 (NP_001614.3).

Sequence

MSQTRDLQGGKAFGLLKAQQEERLDEINKQFLDDPKYSSDEDLPSKLEGFKEKYMEFDLNGNGDIDIMSLKRMLEKLGVPKTHLELKKLIGEVSSGSGETFSYPDFLRMMLGKRSAILKMILMYEEKAREKEKPTGPPAKKAISELP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

17 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AIF-1, AIF1, Allograft inflammatory factor 1, G1, Ionized calcium-binding adapter molecule 1, Protein G1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-Iba1 Antibody (A13488)

Western blot analysis of THP-1, using Anti-Iba1 Antibody (A13488) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Immunohistochemistry - Anti-Iba1 Antibody (A13488)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-Iba1 Antibody (A13488) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Iba1 Antibody (A13488)

Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-Iba1 Antibody (A13488) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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