Western Blot - Anti-HuR / ELAVL1 Antibody (A92898)
Western blot analysis of extracts of various cell lines, using Anti-HuR / ELAVL1 Antibody (A92898) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 20s.
Western Blot - Anti-HuR / ELAVL1 Antibody (A92898)
Western blot analysis of extracts from normal (control) and HuR/ELAVL1 knockout (KO) 293T cells, using Anti-HuR / ELAVL1 Antibody (A92898) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded human esophagus using Anti-HuR / ELAVL1 Antibody (A92898) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human stomach using Anti-HuR / ELAVL1 Antibody (A92898) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-HuR / ELAVL1 Antibody (A92898) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of U2OS cells using Anti-HuR / ELAVL1 Antibody (A92898) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
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