Recombinant Anti-Human IgM Antibody [RM121] (A121348)

Name: Anti-Human IgM Antibody [RM121]
Brand: Antibodies.com
SKU: A121348
Price: 495.0 USD
Availability: InStock

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$495

Recombinant rabbit monoclonal [RM121] antibody to Human IgM for ICC, IHC, Flow Cytometry and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 4-6 business days.

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Recombinant Antibody

Specifications

Name

Anti-Human IgM Antibody [RM121]

Description

Recombinant rabbit monoclonal [RM121] antibody to Human IgM.

Specificity

This antibody recognizes the heavy chain of human IgM.

Applications

ICC, IHC, Flow Cytometry, ELISA

Dilutions

ELISA: 50-200 ng/well (Capture) and 0.05-0.2 µg/ml (Detection), ICC: 0.5-2 µg/ml, IHC: 0.5-2 µg/ml

Reactivity

Human

Cross Reactivity

There is no cross reactivity with human IgG, IgA, IgD, or IgE.

Immunogen

Human IgM.

Host

Rabbit

Clonality

Monoclonal

Clone ID

RM121

Isotype

IgG

Conjugate

Unconjugated

Purification

Protein A chromatography.

Concentration

1 mg/ml

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Ig mu chain C region, Ig mu chain C region BOT, Ig mu chain C region GAL, Ig mu chain C region OU, IGHM, Immunoglobulin heavy constant mu

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Anti-Human IgM Antibody [RM121] (A121348)

Sandwich ELISA using Anti-Human IgM Antibody [RM121] as the capture antibody (100ng/well), and Anti-Human Ig Light Chain Antibody [RM129] (Biotin) as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.

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Anti-Human IgM Antibody [RM121] (A121348)

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Anti-Human IgM Antibody [RM121] (A121348)

ELISA of human immunoglobulins shows Anti-Human IgM Antibody [RM121] reacts only to human IgM. There is no cross reactivity with Human IgG, IgE, IgD, or IgA. The plate was coated with 50ng/well of different immunoglobulins. 200ng/ml or 50ng/ml of Anti-Human IgM Antibody [RM121] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

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Anti-Human IgM Antibody [RM121] (A121348)

A titer ELISA using Anti-Human IgM Antibody [RM121]. The plate was coated with different amounts of human IgM. A serial dilution of Anti-Human IgM Antibody [RM121] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

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