Recombinant Anti-Human IgG1 Antibody [RM117] (A121337)

Name: Anti-Human IgG1 Antibody [RM117]
Brand: Antibodies.com
SKU: A121337
Price: 480.0 USD
Availability: InStock

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$480

Recombinant rabbit monoclonal [RM117] antibody to Human IgG1 for ICC, IHC, Flow Cytometry and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 4-6 business days.

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Recombinant Antibody

Specifications

Name

Anti-Human IgG1 Antibody [RM117]

Description

Recombinant rabbit monoclonal [RM117] antibody to Human IgG1.

Specificity

This antibody recognizes the heavy chain of human IgG1.

Applications

ICC, IHC, Flow Cytometry, ELISA

Dilutions

ELISA: 50-200 ng/well (Capture) and 0.05-0.2 µg/ml (Detection), ICC: 0.5-2 µg/ml, IHC: 0.5-2 µg/ml

Reactivity

Human

Cross Reactivity

There is no cross reactivity with human IgG2, IgG3, IgG4, IgM, IgA, IgD, IgE, monkey IgG, mouse IgG, rat IgG, or goat IgG.

Immunogen

Synthetic peptide corresponding to the hinge region of human IgG1.

Host

Rabbit

Clonality

Monoclonal

Clone ID

RM117

Isotype

IgG

Conjugate

Unconjugated

Purification

Protein A chromatography.

Concentration

1 mg/ml

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (6)

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Anti-Human IgG1 Antibody [RM117] (A121337)

Detection of human IgG1 in monkey serum, using Anti-Human IgG1 Antibody [RM117] (capture antibody) and Anti-Human Ig Light Chain Antibody [RM129] (Biotin) (detection antibody) as a Sandwich ELISA pair. HRP conjugated streptavidin and TMB were used to yield the colorimetric reaction.

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Anti-Human IgG1 Antibody [RM117] (A121337)

Sandwich ELISA using Anti-Human IgG1 Antibody [RM117] as the capture antibody, and Anti-Human Ig Light Chain Antibody [RM129] (Biotin) as the detection antibody, followed by an AP conjugated streptavidin.

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Anti-Human IgG1 Antibody [RM117] (A121337)

ELISA showing Anti-Human IgG1 Antibody [RM117] reacts only to human IgG1, and not to any other IgG subclasses (IgG2, IgG3, or IgG4), and there is no cross reactivity to IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.

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Anti-Human IgG1 Antibody [RM117] (A121337)

A titer ELISA using Anti-Human IgG1 Antibody [RM117]. The plate was coated with different amounts of human IgG1. A serial dilution of Anti-Human IgG1 Antibody [RM117] was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.