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Recombinant Anti-Human IgE Antibody [RM122] (Biotin) (A121279)

ELISA - Anti-Human IgE Antibody (Biotin) [RM122] (A121279) - Antibodies.com ELISA - Anti-Human IgE Antibody (Biotin) [RM122] (A121279) - Antibodies.com ELISA - Anti-Human IgE Antibody (Biotin) [RM122] (A121346) - Antibodies.com
Recombinant Antibody
$480

Recombinant rabbit monoclonal [RM122] antibody to Human IgE (Biotin) for Flow Cytometry and ELISA.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 4-6 business days.

  • Specifications

Name
Anti-Human IgE Antibody [RM122] (Biotin)
Description
Recombinant rabbit monoclonal [RM122] antibody to Human IgE (Biotin).
Specificity
This antibody recognizes human IgE.
Applications
Flow Cytometry, ELISA
Dilutions
ELISA: 10-100 ng/well (Capture) and 0.01-0.1 µg/ml (Detection), ICC: 0.5-2 µg/ml, IHC: 0.5-2 µg/ml
Reactivity
Human
Cross Reactivity
There is no cross reactivity with human IgG, IgM, IgD, or IgA.
Immunogen
Human IgE.
Host
Rabbit
Clonality
Monoclonal
Clone ID
RM122
Isotype
IgG
Conjugate

Biotin

Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Ig epsilon chain C region, Ig epsilon chain C region ND, IGHE, Immunoglobulin heavy constant epsilon
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

ELISA - Anti-Human IgE Antibody (Biotin) [RM122] (A121279) - Antibodies.comEnlarge Image

Anti-Human IgE Antibody [RM122] (Biotin) (A121279)

Sandwich ELISA using Anti-Human IgE Antibody [RM122] as the capture antibody (25ng/well), and Anti-Human Ig Light Chain Antibody [RM129] (Biotin) as the detection antibody, followed by an AP conjugated streptavidin.
ELISA - Anti-Human IgE Antibody (Biotin) [RM122] (A121279) - Antibodies.comEnlarge Image

Anti-Human IgE Antibody [RM122] (Biotin) (A121279)

ELISA of human immunoglobulins shows Anti-Human IgE Antibody [RM122] reacts only to human IgE? from human myeloma plasma and the IgE from human plasma. No cross reactivity with Human IgG, IgM, IgD, or IgA. The plate was coated with 50ng/well of different immunoglobulins. 200ng/ml, 50ng/ml or 10ng/ml of Anti-Human IgE Antibody [RM122] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA - Anti-Human IgE Antibody (Biotin) [RM122] (A121346) - Antibodies.comEnlarge Image

Anti-Human IgE Antibody [RM122] (Biotin) (A121279)

A titer ELISA using Anti-Human IgE Antibody [RM122]. The plate was coated with different amounts of human IgE. A serial dilution of Anti-Human IgE Antibody [RM122] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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