Western Blot - Anti-HSPB1 (phospho S15) Antibody (A16409)
Western blot analysis of extracts of HeLa cells, using Anti-HSPB1 (phospho S15) Antibody (AP0040) at 1:1,000 dilution. HeLa cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 5min.
Immunoprecipitation analysis of 200µg extracts of HeLa cells, using 3µg Anti-HSPB1 (phospho S15) Antibody (AP0040). Western blot was performed from the immunoprecipitate using Anti-HSPB1 (phospho S15) Antibody (AP0040) at a dilition of 1:1000. HeLa cells were treated by EGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight.
Publishing research using Anti-HSPB1 (phospho S15) Antibody (A16409)? Please let us know so that we can list the citation on this page.
Alternative products to Anti-HSPB1 (phospho S15) Antibody (A16409)
![Immunohistochemistry - Anti-HSP27 Antibody [G3.1] (A248872) - Antibodies.com](https://cdn.antibodies.com/image/catalog/248/A248872_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-HSP27 Antibody [8A7] (A304709) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304709_1.png?profile=product_alternative)
![Immunohistochemistry - Anti-HSP27 Antibody [G3.1] - BSA and Azide free (A252052) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252052_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-HSP27 Antibody [SPM252] (A248873) - Antibodies.com](https://cdn.antibodies.com/image/catalog/248/A248873_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-HSP27 Antibody [SPM252] - BSA and Azide free (A252053) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252053_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-HSP27 Antibody [HSPB1/774] (A248874) - Antibodies.com](https://cdn.antibodies.com/image/catalog/248/A248874_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-HSP27 Antibody [HSPB1/774] - BSA and Azide free (A252054) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252054_1.jpg?profile=product_alternative)
![SDS-PAGE - Anti-HSP27 Antibody [CPTC-HSPB1-2] (A248875) - Antibodies.com](https://cdn.antibodies.com/image/catalog/248/A248875_1.jpg?profile=product_alternative)
![Western Blot - Anti-HSP27 Antibody [5D12-A12] (A304734) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304734_1.png?profile=product_alternative)
![SDS-PAGE - Anti-HSP27 Antibody [CPTC-HSPB1-2] - BSA and Azide free (A252055) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252055_1.jpg?profile=product_alternative)