Anti-HSP90 alpha Antibody [Hyb-K41009] (A305061)

Name: Anti-HSP90 alpha Antibody [Hyb-K41009]
Brand: Antibodies.com
SKU: A305061
Price: 540.0 USD
Availability: InStock

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$540

Mouse monoclonal [Hyb-K41009] antibody to HSP90 alpha for WB, IHC and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-HSP90 alpha Antibody [Hyb-K41009]

Description

Mouse monoclonal [Hyb-K41009] antibody to HSP90 alpha.

Specificity

This antibody is alpha specific.

Applications

WB, IHC, ELISA

Dilutions

WB: 1:1,000, IHC: 1:5,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant human HSP90 alpha; Specificity mapped to amino acids 604-731.

Host

Mouse

Clonality

Monoclonal

Clone ID

Hyb-K41009

Isotype

IgG2a

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

90 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.2, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Immunohistochemistry - Anti-HSP90 alpha Antibody [Hyb-K41009] (A305061)

Immunohistochemistry analysis of mouse inflamed colon, fixed in formalin. The Primary Antibody used was Anti-HSP90 alpha Antibody [Hyb-K41009] (A305061) at 1:5,000 for 12 hours at 4°C. The secondary antibody used was Biotin Goat Anti-Mouse at 1:2000 for 1 hour at room temperature. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at room temperature. Localization: Inflammatory cells. Magnification: 40x. Inflammatory cells. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..

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Western Blot - Anti-HSP90 alpha Antibody [Hyb-K41009] (A305061)

Western blot analysis of rat Lysates showing detection of Hsp90 alpha protein using Anti-HSP90 alpha Antibody [Hyb-K41009] (A305061) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.

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Immunohistochemistry - Anti-HSP90 alpha Antibody [Hyb-K41009] (A305061)

Immunohistochemistry analysis of human colon carcinoma, fixed in formalin. The Primary Antibody used was Anti-HSP90 alpha Antibody [Hyb-K41009] (A305061) at 1:5,000 for 12 hours at 4°C. The secondary antibody used was Biotin Goat Anti-Mouse at 1:2000 for 1 hour at room temperature. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at room temperature. Localization: Inflammatory cells. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..

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