Western Blot - Anti-HSP90 alpha Antibody [2G5.G3] (A305031)
Western blot analysis of rat tissue lysate showing detection of Hsp90 alpha protein using Anti-HSP90 alpha Antibody [2G5.G3] (A305031) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.
Immunocytochemistry/Immunofluorescence analysis of human HaCaT cells, fixed in cold 100% methanol for 10 minutes at -20°C, using Anti-HSP90 alpha Antibody [2G5.G3] (A305031), at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature.
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