Western Blot - Anti-HSP70 Antibody [N27F3-4] (A305087)
Western blot analysis of human Cell lysates showing detection of Hsp70 protein using Anti-HSP70 Antibody [N27F3-4] (A305087) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.
Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-HSP70 Antibody [N27F3-4] (A305087) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: Epidermis.
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