Anti-HSP70 Antibody [C92F3A-5] (A304773)

Name: Anti-HSP70 Antibody [C92F3A-5]
Brand: Antibodies.com
SKU: A304773
Price: 535.0 USD
Availability: InStock

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$535

Mouse monoclonal [C92F3A-5] antibody to HSP70 for WB, IHC, ICC/IF, ELISA, Flow Cytometry, FACS, IEM, Block/Inhibit and Antibody Microarray.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Tags:

Cytoplasm Marker ER Marker Mitochondrial Marker Nuclear Marker

Specifications

Name

Anti-HSP70 Antibody [C92F3A-5]

Description

Mouse monoclonal [C92F3A-5] antibody to HSP70.

Applications

WB, IHC, ICC/IF, ELISA, Flow Cytometry, FACS, IEM, Block/Inhibit, Antibody Microarray

Dilutions

WB: 1:1,000, IHC: 1:10,000, ICC/IF: 1:1,000, FACS: 1:1,000

Reactivity

Human, Mouse, Rat, Bovine, C. elegans, Canine, Chicken, Drosophila, Carp, Guinea Pig, Hamster, Monkey, Porcine, Rabbit, Sheep

Cross Reactivity

This antibody does not cross-react with HSC70.

Immunogen

Human HSP70.

Host

Mouse

Clonality

Monoclonal

Clone ID

C92F3A-5

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~70 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.1% Sodium Azide

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Anti-HSP70 Antibody [C92F3A-5] (A304773)

Fluorescence Activated Cell Sorting (FACS) analysis of mouse heat shocked CD3+ CD8+ T cells using Anti-HSP70 Antibody [C92F3A-5] (A304773) at 1:1,000.

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Western Blot - Anti-HSP70 Antibody [C92F3A-5] (A304773)

Western blot analysis of human cell lysates from various cell lines showing detection of Hsp70 protein using Anti-HSP70 Antibody [C92F3A-5] (A304773) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.

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Immunohistochemistry - Anti-HSP70 Antibody [C92F3A-5] (A304773)

Immunohistochemistry analysis of human colon carcinoma, fixed in formalin. The Primary Antibody used was Anti-HSP70 Antibody [C92F3A-5] (A304773) at 1:10,000 for 12 hours at 4°C. The secondary antibody used was Biotin Goat Anti-Mouse at 1:2000 for 1 hour at room temperature. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at room temperature. Localization: Inflammatory cells. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..

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Immunofluorescence - Anti-HSP70 Antibody [C92F3A-5] (A304773)

Immunocytochemistry/Immunofluorescence analysis of mouse heat shocked melanoma cells, fixed in formalin, using Anti-HSP70 Antibody [C92F3A-5] (A304773), at 1:1,000 for 16 hours at room temperature. The secondary antibody used was Biotin Goat Anti-Mouse.