Immunohistochemistry analysis of rat hepatocytes, fixed in Paraffin Embedded. The Primary Antibody used was Anti-HSP70 Antibody [BB70] (A305113) at 1:200. liver sections were paraffin embedded. First pictures in series show two hours after exposure to stress, the second shows the control.
Western Blot - Anti-HSP70 Antibody [BB70] (A305113)
Western blot analysis of human Cervical cancer cell line (HeLa) lysate showing detection of Hsp70 protein using Anti-HSP70 Antibody [BB70] (A305113) at 1:1,000. The secondary antibody used was HRP Goat Anti-Rat.
Immunocytochemistry/Immunofluorescence analysis of rat hepatocyte nuclei, using Anti-HSP70 Antibody [BB70] (A305113), at 1:200. liver sections were paraffin embedded. First pictures in series show two hours after exposure to stress, the second shows the control.
Immunohistochemistry analysis of human colon carcinoma, fixed in formalin. The Primary Antibody used was Anti-HSP70 Antibody [BB70] (A305113) at 1:10,000 for 12 hours at 4°C. The secondary antibody used was Biotin Goat Anti-Mouse at 1:2000 for 1 hour at room temperature. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at room temperature. Localization: Inflammatory cells. Magnification: 40x. HSP70/HSC70 cells stained brown. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..
Immunohistochemistry analysis of mouse inflamed colon, fixed in formalin. The Primary Antibody used was Anti-HSP70 Antibody [BB70] (A305113) at 1:10,000 for 12 hours at 4°C. The secondary antibody used was Biotin Goat Anti-Mouse at 1:2000 for 1 hour at room temperature. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at room temperature. Localization: Inflammatory cells. Magnification: 40x. Inflammatory cells. HSP70/HSC70 stained brown. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..
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