Anti-HSP70 Antibody (A90945)

Name: Anti-HSP70 Antibody
Brand: Antibodies.com
SKU: A90945
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to HSP70 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Tags:

Cytoplasm Marker ER Marker Mitochondrial Marker Nuclear Marker

Specifications

Name

Anti-HSP70 Antibody

Description

Rabbit polyclonal antibody to HSP70.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:1,000-1:3,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 500-600 of human HSP70/HSPA1 (NP_005336.3).

Sequence

KITITNDKGRLSKEEIERMVQEAEKYKAEDEVQRERVSAKNALESYAFNMKSAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELE

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

70 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-HSP70 Antibody (A90945)

Western blot analysis of extracts of various cell lines, using Anti-HSP70 Antibody (A90945) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Immunohistochemistry - Anti-HSP70 Antibody (A90945)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-HSP70 Antibody (A90945) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-HSP70 Antibody (A90945)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-HSP70 Antibody (A90945) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-HSP70 Antibody (A90945)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-HSP70 Antibody (A90945) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.