Western blot analysis of Trypanosoma brucei brucei cells showing detection of ~71 kDa TbHsp70 protein using Anti-HSP70 Antibody (A305237) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: Cells incubated for 1 hour at 37°C. Lane 3: Cells incubated for 1 hour at 42°C. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: ~71 kDa.
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