Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-HSP70 Antibody (A305017), at 1:120 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Chicken (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cytoplasm. Perinuclear region of cytoplasm. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-Hsp70 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-HSP70 Antibody (A305017), at 1:120 for 12 hours at 4°C. The secondary antibody used was R-PE Goat Anti-Chicken (yellow) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cytoplasm. Perinuclear region of cytoplasm. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-Hsp70 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Western blot analysis of human Cervical cancer cell line (HeLa) lysate showing detection of HSP70 protein using Anti-HSP70 Antibody (A305017) at 1:1,000 for 2 hours at room temperature. Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Goat anti-Chicken IgY: HRP for 1 hour at room temperature.
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