Western Blot - Anti-HSP70 Antibody [2A4] (A305075)
Western blot analysis of rat Cell lysates showing detection of Hsp70 protein using Anti-HSP70 Antibody [2A4] (A305075) at 1:5,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.
Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-HSP70 Antibody [2A4] (A305075), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:5,000 for 5 minutes room temperature. Localization: Nucleus, Cytoplasm. Magnification: 40X.
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