Anti-HSP60 Antibody [ARC0260] (A309137)

Name: Anti-HSP60 Antibody [ARC0260]
Brand: Antibodies.com
SKU: A309137
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC0260] antibody to HSP60 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Tags:

Mitochondrial Loading Control Mitochondrial Marker

Specifications

Name

Anti-HSP60 Antibody [ARC0260]

Description

Rabbit monoclonal [ARC0260] antibody to HSP60.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 350-450 of human HSP60/HSPD1 (P10809).

Sequence

VTKDDAMLLKGKGDKAQIEKRIQEIIEQLDVTTSEYEKEKLNERLAKLSDGVAVLKVGGTSDVEVNEKKDRVTDALNATRAAVEEGIVLGGGCALLRCIPA

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0260

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-HSP60 Antibody [ARC0260] (A309137)

Western blot analysis of extracts of various cell lines, using Anti-HSP60 Antibody [ARC0260] (A309137) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Immunohistochemistry - Anti-HSP60 Antibody [ARC0260] (A309137)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-HSP60 Antibody [ARC0260] (A309137) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-HSP60 Antibody [ARC0260] (A309137)

Confocal imaging of HeLa cells using Anti-HSP60 Antibody [ARC0260] (A309137), at a dilution of 1:100, (red). The cells were counterstained with Anti-alpha Tubulin Antibody, at a dilution of 1:100, (green). DAPI was used for nuclear staining (Blue). Objective: 60x.

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