Western blot analysis of extracts of various cell lines, using Anti-HSP60 Antibody (A12728) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-HSP60 Antibody (A12728) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-HSP60 Antibody (A12728) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-HSP60 Antibody (A12728) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Confocal immunofluorescence analysis of HeLa cells using Anti-HSP60 Antibody (A12728) at a dilution of 1:400. DAPI was used to stain the cell nuclei (blue).
Confocal immunofluorescence analysis of U-2 OS cells using Anti-HSP60 Antibody (A12728) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Immunoprecipitation analysis of 200µg extracts of HeLa cells using 1µg of Anti-HSP60 Antibody (A12728). This Western blot was performed on the immunoprecipitate using Anti-HSP60 Antibody (A12728) at a dilution of 1:1000.
![Immunocytochemistry/Immunofluorescence - Anti-HSP60 Antibody [LK1] (A305088) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305088_1.png?profile=product_alternative)
![Immunofluorescence - Anti-HSP60 Antibody [LK2] - BSA and Azide free (A252064) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252065_1.jpg?profile=product_alternative)
![Immunofluorescence - Anti-HSP60 Antibody [LK2] (A248884) - Antibodies.com](https://cdn.antibodies.com/image/catalog/248/A248885_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-HSP60 Antibody [LK2] (A305090) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305090_1.png?profile=product_alternative)
![Immunohistochemistry - Anti-HSP60 Antibody [HSPD1/780] (A248887) - Antibodies.com](https://cdn.antibodies.com/image/catalog/248/A248887_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-HSP60 Antibody [LK1] (A248883) - Antibodies.com](https://cdn.antibodies.com/image/catalog/248/A248883_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-HSP60 Antibody [HSPD1/780] - BSA and Azide free (A252067) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252067_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-HSP60 Antibody [LK1] - BSA and Azide free (A252063) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252063_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-HSP60 Antibody [SPM253] (A248884) - Antibodies.com](https://cdn.antibodies.com/image/catalog/248/A248884_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-HSP60 Antibody [SPM253] - BSA and Azide free (A252064) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252064_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-HSP60 Antibody [rGROEL/780] - BSA and Azide free (A252069) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252069_1.jpg?profile=product_alternative)