Anti-HSP60 Antibody [1C7] (A85439)

Name: Anti-HSP60 Antibody [1C7]
Brand: Antibodies.com
SKU: A85439
Price: 190.0 USD
Availability: InStock

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$190

Mouse monoclonal [1C7] antibody to HSP60 for WB, ICC/IF and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Tags:

Mitochondrial Loading Control Mitochondrial Marker

Specifications

Name

Anti-HSP60 Antibody [1C7]

Description

Mouse monoclonal [1C7] antibody to HSP60.

Applications

WB, ICC/IF, IHC

Dilutions

WB: 1:10,000, ICC/IF: 1:5,000, IHC: 1:5,000

Reactivity

Human, Rat, Mouse

Immunogen

Spontaneous autoantibody which binds LNERLAKLSDGVAVLKVGGT, amino acids 390-409 of the human sequence.

Host

Mouse

Clonality

Monoclonal

Clone ID

1C7

Isotype

IgG1

Conjugate

Unconjugated

Purification

Immunogen affinity purification.

Concentration

1 mg/ml

Molecular Weight

60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 5mM Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

General Notes

This antibody was discovered during screens for antibodies produced by hybridomas from a mouse injected with an unrelated protein. We noted beautiful staining of mitochondria and clean staining of a single band of 60 kDa on western blots, suggesting HSP60 as an obvious candidate. We made recombinant full-length human HSP60 which this antibody bound to strongly and specifically. The mouse had likely developed autoantibodies to HSP60 since this molecule appears to be unusually immunogenic, frequently generating autoantibodies in humans and other species.

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-HSP60 Antibody [1C7] (A85439)

Western blot analysis of tissue or whole cell lysates using Anti-Heat Shock Protein 60 Antibody, at a dilution of 1:10,000, in green,: [Lane 1] protein standard (red), [Lane 2] rat brain, [Lane 3] mouse brain, [Lane 4] NIH/3T3, [Lane 5] Hek 293, [Lane 6] HeLa, [Lane 7] SH-SY5Y cells. Strong single band corresponds to HSP60 protein with apparent SDS-PAGE molecular weight of 60 kDa.

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Immunofluorescence - Anti-HSP60 Antibody [1C7] (A85439)

Immunofluorescent analysis of HeLa cells stained with Anti-Heat Shock Protein 60 Antibody [1C7] (A85439), at a dilution of 1:5,000, in red. Blue is DAPI staining of nuclear DNA. Anti-Heat Shock Protein 60 Antibody [1C7] (A85439) produces strong and specific staining of mitochondria.

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Immunohistochemistry - Anti-HSP60 Antibody [1C7] (A85439)

Immunohistochemistry analysis of a 4% PFA fixed paraffin embedded rat cerebellum section with Anti-HSP60 Antibody [1C7] (A85439) at a dilution of 1:1,000 detected with DAB (brown) using the ImmPress method, with citra buffer retrieval. Counterstained with Hematoxylin (blue). Note: this antibody performs well in testing with both 4% PFA and standard NBF fixed tissues but does not stain long term NBF fixed tissue effectively.

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Western Blot - Anti-HSP60 Antibody [1C7] (A85439)

Western blot analysis of tissue or whole cell lysates using Anti-HSP60 Antibody [1C7] (A85439) at a dilution of 1:10,000, (green). [1] protein standard (red), [Lane 2] rat brain, [Lane 3] mouse brain, [Lane 4] NIH-3T3, [Lane 5] HEK293, [Lane 6] HeLa, and [Lane 7] SH-SY5Y cells. Strong single band corresponds to HSP60 protein with apparent SDS-PAGE molecular weight of 60kDa.