Anti-Hsp47 Antibody [ARC0681] (A80870)

Name: Anti-Hsp47 Antibody [ARC0681]
Brand: Antibodies.com
SKU: A80870
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC0681] antibody to Hsp47 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Hsp47 Antibody [ARC0681]

Description

Rabbit monoclonal [ARC0681] antibody to Hsp47.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 319-418 of human HSP47/SERPINH1 (P50454).

Sequence

KHLAGLGLTEAIDKNKADLSRMSGKKDLYLASVFHATAFELDTDGNPFDQDIYGREELRSPKLFYADHPFIFLVRDTQSGSLLFIGRLVRPKGDKMRDEL

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0681

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

46 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

47 kDa heat shock protein, Arsenic-transactivated protein 3, AsTP3, CBP1, CBP2, Cell proliferation-inducing gene 14 protein, Collagen-binding protein, Colligin, Rheumatoid arthritis-related antigen RA-A47, Serpin H1, SERPINH1, SERPINH2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-Hsp47 Antibody [ARC0681] (A80870)

Western blot analysis of extracts of various cell lines, using Anti-Hsp47 Antibody [ARC0681] (A80870) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Immunohistochemistry - Anti-Hsp47 Antibody [ARC0681] (A80870)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-Hsp47 Antibody [ARC0681] (A80870) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-Hsp47 Antibody [ARC0681] (A80870)

Immunofluorescence analysis of NIH-3T3 cells using Anti-Hsp47 Antibody [ARC0681] (A80870) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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