HSP47 expression in various lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-HSP47 Antibody (A90026) at a dilution of 1:1000 and detected with chemiluminescence.
HSP47 expression in NIH/3T3 cells analyzed by immunofluorescence. Staining was performed with Anti-HSP47 Antibody (A90026) at a dilution of 1:100 followed by Cy3-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).
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