Western Blot - Anti-Hsp27 (phospho Ser82) Antibody [ARC1576] (A308347)
Western blot analysis of extracts of HeLa cells, using Anti-Hsp27 (phospho Ser82) Antibody [ARC1576] (A308347) at 1:1,000 dilution. HeLa cells were treated by UV at room temperature for 15-30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 3s.
Western Blot - Anti-Hsp27 (phospho Ser82) Antibody [ARC1576] (A308347)
Western blot analysis of extracts of PC-12 cells, using Anti-Hsp27 (phospho Ser82) Antibody [ARC1576] (A308347) at 1:1,000 dilution. PC-12 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour or treated by UV at room temperature for 15-30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 90s.
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