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Anti-HSP27 (phospho Ser82) Antibody (A94054)

Western Blot - Anti-HSP27 (phospho Ser82) Antibody (A7112) - Antibodies.com Western Blot - Anti-HSP27 (phospho Ser82) Antibody (A7112) - Antibodies.com
$190
Product Datasheet

Rabbit polyclonal antibody to HSP27 (phospho Ser82) for WB and ELISA.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.

  • Specifications

Name
Anti-HSP27 (phospho Ser82) Antibody
Description
Rabbit polyclonal antibody to HSP27 (phospho Ser82).
Specificity
This antibody detects endogenous levels of HSP27 only when phosphorylated at Ser82.
Applications
WB, ELISA
Dilutions
WB: 1:500-1:1000, ELISA: 1:4000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide derived from human HSP27 around the phosphorylation site of Ser82 (amino acids 48-97).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
22kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Heat shock protein beta-1, Heat shock protein family B member 1, HSP 27, HSP28, HspB1, SRP27, Stress-responsive protein 27
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-HSP27 (phospho Ser82) Antibody (A7112) - Antibodies.comEnlarge Image

Western Blot - Anti-HSP27 (phospho Ser82) Antibody (A94054)

Western blot analysis of lysates from HUVEC cells treated with TNF 20ng/ml 30' using Anti-HSP27 (phospho Ser82) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Western Blot - Anti-HSP27 (phospho Ser82) Antibody (A7112) - Antibodies.comEnlarge Image

Western Blot - Anti-HSP27 (phospho Ser82) Antibody (A94054)

Western blot analysis of various cells using Anti-HSP27 (phospho Ser82) Antibody.
Publishing research using Anti-HSP27 (phospho Ser82) Antibody (A94054)? Please let us know so that we can list the citation on this page.

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