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Anti-Hsp27 (phospho Ser78) Antibody [ARC1577] (A305364)

Western Blot - Anti-Hsp27 (phospho Ser78) Antibody [ARC1577] (A305364) - Antibodies.com
$560
Product Datasheet

Rabbit monoclonal [ARC1577] antibody to Hsp27 (phospho Ser78) for WB.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.

  • Specifications

Name
Anti-Hsp27 (phospho Ser78) Antibody [ARC1577]
Description
Rabbit monoclonal [ARC1577] antibody to Hsp27 (phospho Ser78).
Applications
WB
Dilutions
WB: 1:500-1:2,000
Reactivity
Human, Mouse
Immunogen
A synthetic phosphorylated peptide around S78 of human HSP27/HSPB1HSPB1 (P04792).
Sequence
ALSRQ
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC1577
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
27 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Heat shock protein beta-1, Heat shock protein family B member 1, HSP 27, HSP28, HspB1, SRP27, Stress-responsive protein 27
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Western Blot - Anti-Hsp27 (phospho Ser78) Antibody [ARC1577] (A305364) - Antibodies.comEnlarge Image

Western Blot - Anti-Hsp27 (phospho Ser78) Antibody [ARC1577] (A305364)

Western blot analysis of extracts of HeLa cells, using Anti-Hsp27 (phospho Ser78) Antibody [ARC1577] (A305364) at 1:1,000 dilution. HeLa cells were treated by UV at room temperature for 15-30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 3s.
Publishing research using Anti-Hsp27 (phospho Ser78) Antibody [ARC1577] (A305364)? Please let us know so that we can list the citation on this page.

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