Anti-HSP27 (Phospho-Ser78) Antibody (A50197)

Name: Anti-HSP27 (Phospho-Ser78) Antibody
Brand: Antibodies.com
SKU: A50197
Price: 275.0 USD
Availability: InStock

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$275

Rabbit polyclonal antibody to HSP27 (Phospho-Ser78) for WB, IHC and IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 6-9 business days.

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Cytoskeleton Marker

Specifications

Name

Anti-HSP27 (Phospho-Ser78) Antibody

Description

Rabbit polyclonal antibody to HSP27 (Phospho-Ser78)

Specificity

The antibody detects endogenous level of HSP27 only when phosphorylated at serine 78.

Applications

WB, IHC, IF

Reactivity

Human

Immunogen

Peptide sequence around phosphorylation site of serine 78 (A-L-S(p)-R-Q) derived from Human HSP27.

Host

Rabbit

Clonality

Polyclonal

Conjugate

Unconjugated

Purification

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/ml

Formulation

Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Synonyms

28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Heat shock protein beta-1, Heat shock protein family B member 1, HSP 27, HSP28, HspB1, SRP27, Stress-responsive protein 27

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Anti-HSP27 (Phospho-Ser78) Antibody (A50197)

Western blot analysis of extracts from HL60 cells untreated or treated with UV using HSP27 (Phospho-Ser78) Antibody #11247.

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Immunohistochemistry - Anti-HSP27 (Phospho-Ser78) Antibody (A50197)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27 (Phospho-Ser78) Antibody #11247 (left) or the same antibody preincubated with blocking peptide (right).

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Immunofluorescence - Anti-HSP27 (Phospho-Ser78) Antibody (A50197)

Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic, nuclear, centrosomal, midbody staining using HSP27 (Phospho-Ser78) Antibody #11247.

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