Western blot analysis of extracts of various cell lines, using Anti-Hsp27 Antibody (A307909) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunoprecipitation analysis of 200µg extracts of HepG2 cells using 3µg of Anti-Hsp27 Antibody (A307909). This Western blot was performed on the immunoprecipitate using Anti-Hsp27 Antibody (A307909) at a dilution of 1:1000.
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