Western blot analysis of rat tissue mix showing detection of HSPB2 protein using Anti-Hsp27 Antibody (A304984) at 1:1,000 for 2 hours at room temperature. Load: 15 µgprotein. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Donkey Anti-Rabbit IgG: HRP for 1 hour at room temperature.
Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-Hsp27 Antibody (A304984), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Nucleus. Cytoplasm. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-HSPB2 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
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