Anti-HSP27 Antibody (A12566)

Name: Anti-HSP27 Antibody
Brand: Antibodies.com
SKU: A12566
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to HSP27 for WB, IHC-P and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

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Cytoskeleton Marker

Specifications

Name

Anti-HSP27 Antibody

Description

Rabbit polyclonal antibody to HSP27.

Applications

WB, IHC-P, ELISA

Positive Control

HepG2, HeLa, mouse lung, rat lung.

Dilutions

WB: 1:500-1:2,000, IHC-P: 1:1,000-1:5,000, ELISA: 1 µg/ml (starting concentration)

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant fusion protein corresponding to amino acids 1-100 of human HSP27.

Sequence

MTERRVPFSLLRGPSWDPFRDWYPHSRLFDQAFGLPRLPEEWSQWLGGSSWPGYVRPLPPAAIESPAVAAPAYSRALSRQLSSGVSEIRHTADRWRVSLD

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

25 kDa

Predicted MW

23 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Heat shock protein beta-1, Heat shock protein family B member 1, HSP 27, HSP28, HspB1, SRP27, Stress-responsive protein 27

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-HSP27 Antibody (A12566)

HSP27 expression in HepG2 cell lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-HSP27 Antibody (A12566) at a dilution of 1:1000 and detected with chemiluminescence.

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Western Blot - Anti-HSP27 Antibody (A12566)

HSP27 expression in Rat lung cell lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-HSP27 Antibody (A12566) at a dilution of 1:1000 and detected with chemiluminescence.

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Western Blot - Anti-HSP27 Antibody (A12566)

HSP27 expression in HeLa cell lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-HSP27 Antibody (A12566) at a dilution of 1:1000 and detected with chemiluminescence.

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Western Blot - Anti-HSP27 Antibody (A12566)

HSP27 expression in Mouse lung lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-HSP27 Antibody (A12566) at a dilution of 1:1000 and detected with chemiluminescence.