Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-HSP27 Antibody [8A7] (A304709) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: Epidermis.
Western Blot - Anti-HSP27 Antibody [8A7] (A304709)
Western blot analysis of rat lung tissue lysates showing detection of Hsp27 protein using Anti-HSP27 Antibody [8A7] (A304709) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 5% blocking solution. The secondary antibody used was Goat Anti-Mouse: HRP for 1 hour at room temperature.
Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-HSP27 Antibody [8A7] (A304709) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: Epidermis.
Immunohistochemistry analysis of human colon carcinoma, fixed in formalin. The Primary Antibody used was Anti-HSP27 Antibody [8A7] (A304709) at 1:5,000 for 12 hours at 4°C. The secondary antibody used was Biotin Goat Anti-Mouse at 1:2000 for 1 hour at room temperature. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at room temperature. Localization: Inflammatory cells. Magnification: 40x.
Immunohistochemistry analysis of rat embryo somites. The Primary Antibody used was Anti-HSP27 Antibody [8A7] (A304709) at 1:1,000. The secondary antibody used was FITC Goat Anti-Mouse (green). Counterstain: Rhodamine-phalloidin labeled actin (red). Investigation at 11 days gestation.
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